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Пептивет Ото Гель - оценка эффективности in vitro

Пептивет Ото Гель - оценка эффективности in vitro


Оценка эффективности in vitro коммерческого отологического раствора ,содержащего новый антимикробный пептид  AMP2041 в отношении 30 клинических изолятов Pseudomonas aeruginosa, выделенных от собак с наружным отитом

    

G. Ghibaudo 1,*, D. Santospirito 2, A. Sala 2, S. Flisi 2, S. Taddei 2, S. C. Cabassi 2

1 Ветеринарная клиника Мальпенза, Самарат, Италия, 2 Департамент Ветеринарной медицины, Университет Пармы, Италия

 

Введение

У собак инфекции Pseudomonas aeruginosa (PA) часто проявляются в качестве острого гнойного отита с сильным воспалением, изъязвлением, дискомфортом и болью. Это следует рассматривать как терапевтический вызов, требующий нового эффективного лечения.

Задачи

Целью данного исследования было оценить антимикробную активность in vitro коммерческого отологического раствора (Peptivet® oto., ICF, Cremona, Italy), содержащего 0,02% диглюконата хлоргексидина (CLX), 0,4% трис, 0,1% EDTA и   0,5 мкг / мл антимикробного пептида AMP2041 на изоляты Pseudomonas aeruginosa ,выделенные от собак с  отитом.

Материалы и методы

Минимальные бактерицидные концентрации (MБК) оценивали путем микроразведений бульона. Стандартизованные бактериальные суспензии инкубировали с различными концентрациями испытуемого раствора при рН 8 и 37 ° С в течение 30 мин, а затем распределяли по размерам покрытия среды для подсчета колониеобразующих единиц (КОЕ). Динамику гибели бактерий оценивали по кривым времени, полученному при использовании неразбавленного продукта, и при MБК для каждого штамма путем аликвотации и покрытия 20 мкл бактериальной суспензии для подсчета КОЕ  через фиксированные интервалы времени (30 с, 1, 5, 10, 15 мин , 24ч и 48ч).

Результаты

Минимальная бактерицидная концентрация (МБК) составила 1:32 для 1 из 30 штаммов и 1:64 для 29/30 штаммов. Среднее геометрическое значение составило 1: 62,54, что эквивалентно концентрации 0,0003% Хлоргексидина/ 0,0064% Tris / 0,0016% ЭДТА / 0,008 мкг / мл AMP2041. Тесты на скорость уничтожения бактерий с неразбавленным продуктом показали полный бактерицидный эффект в течение 5 минут для всех изолятов, тогда как при MБК этот эффект был достигнут в течение 10 минут для 28 изолятов из 30. Полная бактерицидный эффект наблюдался через 48 часов для всех штаммов

ВЫВОДЫ: Продукт демонстрирует быструю, полную и продолжительную антимикробную активность против группы из 30 клинических изолятов Pseudomonas aeruginosa от собак с  отитом .





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